Matias Attene Ramos TabsBio Dr. Attene Ramos’ main research interest lies in understanding how chemicals can perturb biological systems leading to the onset and progression of different environmental diseases, and how those systems respond to maintain homeostasis. His ultimate goal is to use a mechanistic, disease based approach to improve toxicity testing and chemical hazard characterization using computational, biochemical, and cellular models to reduce animal use in toxicology. Dr. Attene Ramos was a fellow at the NIH Chemical Genomics Center where he worked for the tox21 high throughput initiative under the National Toxicology Program to accelerate the hazard characterization of thousands of compounds present in the environment. He received his PhD from the University of Illinois at Urbana-Champaign ExpertiseExpertise: Environmental and Occupational HealthGlobal Environmental HealthRisk Assessment, Management and CommunicationWater, Sanitation, HygieneToxic wasteEducation: Postdoctoral fellow, NIH (NHGRI/NCATS), 2013 PhD, University of Illinois at Urbana-Champaign, 2008 Teaching: PubH 6015.10 Culminating Experience ResearchResearch: Dr. Attene Ramos’ research projects include: Develop disease focused toxicity testing methods to accelerate hazard characterization of environmental chemicals. Define the role of environmental chemicals in mitochondrial diseases. Understand the potential toxic effects of chemicals generated during water disinfection, and water reuse. Use these information to optimize those processes. Generate predictive models for complex mixture toxicity. Use a combined computational/experimental approach to optimize computational models capable of predicting reactive toxicity. PublicationsPublications: Sakamuru, S, Attene-Ramos, MS, Xia, M. Mitochondrial membrane potential assay. Methods in Mol Biol 1473, 17-22 (2016). Huang R, Xia M, Sakamuru S, Zhao J, Shahane SA, Attene-Ramos MS, Zhao T, Austin CP, Simeonov A. Modelling the Tox21 10 K chemical profiles for in vivo toxicity prediction and mechanism characterization. Nat Communications. 7:10425 (2016). Attene-Ramos MS, Huang R, Michael S, Witt KL, Tice RR, Austin CP, and Xia M. Profiling of the Tox21 chemical collection for mitochondrial function to identify compounds that acutely decrease mitochondrial membrane potential. Environmental Health Perspectives, 123:49–56 (2015). 20 Foley TL, Rai G, Yasgar A, Daniel T, Baker HL, Attene-Ramos M, Kosa NM, Leister W, Burkart MD, Jadhav A, Simeonov A, Maloney DJ. 4-(3-Chloro-5-(trifluoromethyl)pyridin-2-yl)-N-(4-methoxypyridin-2-yl)piperazine-1-carbothioamide (ML267), a potent inhibitor of bacterial Phosphopantetheinyl Transferase that attenuates secondary metabolism and thwarts bacterial growth. Journal of Medicinal Chemistry, 13;57:1063-78 (2014). Foley TL, Rai G, Yasgar A, Attene-Ramos MS, Burkart MD, Simeonov A, Jadhav A, Maloney DJ. Discovery of ML 267 as a novel inhibitor of pathogenic Sfp phosphopantetheinyl transferase (PPTase). Probe Reports from the NIH Molecular Libraries Program Bethesda (MD): National Center for Biotechnology Information (US). 2010-.2012 (2013). Pals J, Attene-Ramos MS, Xia M, Wagner ED, Plewa MJ. Human cell toxicogenomic analysis linking reactive oxygen species to the toxicity of monohaloacetic Acid drinking water disinfection byproducts. Environmental Science & Technology, 5;47:12514-23 (2013). Attene-Ramos MS, Huang R, Sakamuru S, Witt KL, Beeson GC, Shou L, Schnellmann RG, Beeson CC, Tice RR, Austin CP, Xia M. Systematic study of mitochondrial toxicity of environmental chemicals using quantitative high throughput screening. Chemical Research in Toxicology, 26:1323-32 (2013). Attene-Ramos MS, Miller N, Huang R, Michael S, Itkin M, Kavlock RJ, Austin CP, Shinn P, Simeonov A, Tice RR, Xia M. The Tox21 robotic platform for the assessment of environmental chemicals - from vision to reality. Drug Discovery Today, 18:716-23 (2013). Sakamuru S, Li X, Attene-Ramos MS, Huang R, Lu J, Shou L, Shen M, Tice RR, Austin CP, Xia M. Application of a homogenous membrane potential assay to assess mitochondrial function. Physiological Genomics, 44:495-503 (2012). Escorcia M, Attene-Ramos MS, Estrada MJ, Nava GM. Improving global influenza surveillance: trends of A(H5N1) virus in Africa and Asia. BMC Research Notes, 23;5:62. (2012). Huang W, Huang R, Attene-Ramos MS, Sakamuru S, Englund EE, Inglese J, Austin CP, Xia M. Synthesis and evaluation of quinazolin-4-ones as hypoxia-inducible factor-1α inhibitors. Bioorganic and Medicinal Chemistry Letters, 15;21:5239-43 (2011). Duirk SE, Lindell C, Cornelison C, Kormos JL, Ternes TA, Attene-Ramos MS, Osiol J, Wagner ED, Plewa MJ, Richardson SD. Formation of toxic iodinated disinfection by-products from compounds used in medical imaging. Environmental Science & Technology, 15;45:6845-54 (2011). Attene-Ramos MS, Wagner ED, Plewa MJ. Comparative Human Cell Toxicogenomic Analysis of Monohaloacetic Acid Drinking Water Disinfection Byproducts. Environmental Science & Technology, 44:7206-12 (2010). Attene-Ramos MS, Nava GM, Muellner MG, Wagner ED, Plewa MJ, Gaskins HR. DNA damage and toxicogenomic analysis of hydrogen sulfide in human intestinal epithelial FHs 74 Int cells. Environmental and Molecular Mutagenesis, 51:304-14 (2010). Miller SC, Huang R, Sakamuru S, Shukla SJ, Attene-Ramos MS, Shinn P, Van Leer D, Leister W, Austin CP, Xia M. Identification of known drugs that act as inhibitors of NF-kappa B signaling and their mechanism of action. Biochemical Pharmacology, 79:1272-80 (2010). Muellner MG, Attene-Ramos MS, Hudson ME, Wagner ED, Plewa MJ. Human cell toxicogenomic analysis of bromoacetic acid: A regulated drinking water disinfection by-product. Environmental and Molecular Mutagenesis, 51:205-14 (2009). Nava GM, Attene-Ramos MS, Ang JK, Escorcia M. Origins of the new influenza A(H1N1) virus: time to take action. Eurosurveillance, 14:19228 (2009). Nava GM, Attene-Ramos MS, Gaskins HR, Richards JD. Molecular analysis of microbial community structure in the chicken ileum following organic acid supplementation. Veterinary Microbiology, 137:345-53 (2009). Attene-Ramos MS, Wagner ED, Gaskins HR, Plewa MJ. Hydrogen sulfide induces direct radical-associated DNA damage. Molecular Cancer Research, 5:455-459 (2007). Marini JC, Attene-Ramos MS. An improved analytical method for the determination of urea nitrogen isotopomers in biological samples utilizing continuous flow isotope ratio mass spectrometry. Rapid Communications in Mass Spectrometry, 20:3736-40 (2006). Attene-Ramos MS, Wagner ED, Plewa MJ, Gaskins HR. Evidence that hydrogen sulfide is a genotoxic agent. Molecular Cancer Research, 4:9-14 (2006). Attene-Ramos MS, Kitiphongspattana K, Ishii-Schrade K, Gaskins HR. Temporal changes of multiple redox couples from proliferation to growth arrest in IEC-6 intestinal epithelial cells. American Journal of Physiology Cell Physiology, 289:1220-8 (2005). Graham WV, Tcheng DK, Shirk AL, Attene-Ramos MS, Welge ME, Gaskins HR. Phylomat: an automated protein motif analysis tool for phylogenomics. Journal of Proteome Research, 3:1289-91 (2004).